Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), eBioscience
인간 MHC class I(HLA-A, B, C)를 인식하는 W6/32 모노클로날 항체로, 세포면 항원 분석 및 면역학 연구에 적합. Flow cytometry, IHC(F), IP 등 다양한 응용 가능. 0.5 mg/mL 액상 형태, 4°C 보관. 인간 및 영장류 시료에 교차 반응.
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Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 2 |
| Immunocytochemistry (ICC/IF) | - | 3 |
| Flow Cytometry (Flow) | 1 µg/test | 22 |
| ELISA | - | 2 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 |
| Functional Assay | - | 5 |
| Inhibition Assays | - | 3 |
| In vitro Assay | - | 2 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| Item | Description |
|---|---|
| Published Species | Cynomolgus monkey, Fruit fly, Human, Mouse, Virus |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | W6/32 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468660 |
Available Conjugated Formats
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395, 615, 737
- Brilliant Violet 650, 786
- FITC
- Functional Grade
- NovaFluor Blue 510, 610-70S, 660-120S, 690
- NovaFluor Red 710, 725, 755
- NovaFluor Yellow 590, 660, 755
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 702
- Custom conjugation available upon request
Product Specific Information
The W6/32 monoclonal antibody reacts with human major histocompatibility complex (MHC) class I, HLA-A, B, and C. These antigens, associated with beta2-microglobulin, are expressed by all nucleated cells and play a key role in cell-mediated immune response and tumor surveillance.
W6/32 mAb recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.
Applications Reported: Flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells (≤1 µg/test). A test is defined as the amount of antibody that stains a cell sample in a 100 µL final volume (typically 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
HLA-class I MHC antigens are intrinsic membrane glycoproteins expressed on nucleated cells, noncovalently associated with beta2-microglobulin. They present foreign determinants for immune recognition by cytotoxic T cells, essential for antiviral and antitumor defense.
Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.
HLA and MHC antibodies are critical in Immunopeptidomics, enabling identification and characterization of neoantigens via HPLC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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