Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), eBioscience

Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395, 615, 737
• Brilliant Violet 650, 786
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-70S, 660-120S, 690
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 590, 660, 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 702
• Custom conjugation available upon request

Product Specific Information

The W6/32 monoclonal antibody reacts with human major histocompatibility complex (MHC) class I, HLA-A, B, and C. These antigens, associated with beta2-microglobulin, are expressed by all nucleated cells and play a key role in cell-mediated immune response and tumor surveillance.
W6/32 mAb recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.

Applications Reported: Flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells (≤1 µg/test). A test is defined as the amount of antibody that stains a cell sample in a 100 µL final volume (typically 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-class I MHC antigens are intrinsic membrane glycoproteins expressed on nucleated cells, noncovalently associated with beta2-microglobulin. They present foreign determinants for immune recognition by cytotoxic T cells, essential for antiviral and antitumor defense.
Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.

HLA and MHC antibodies are critical in Immunopeptidomics, enabling identification and characterization of neoantigens via HPLC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.