Thermo Fisher Scientific Epsin 2 Polyclonal Antibody
Epsin 2 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, ICC/IF에 사용 가능. 인간, 생쥐, 랫트 반응성. 친화 크로마토그래피 정제 및 PBS/glycerol 보존. 세포 내 클라트린 매개 엔도사이토시스 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EPN2 (Accession O95208), corresponding to amino acid residues P386–K436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818230 |
Product Specific Information
This antibody detects endogenous levels of total EPN2.
Target Information
Epsin2 plays a role in the formation of clathrin-coated invaginations and endocytosis. It binds to EPS15 (via the NPF repeat domain), as well as to AP-2 and clathrin (via the DPW repeat domain). The protein resides in the cytoplasm, in punctate structures throughout the cell, associated with clathrin-coated vesicles, and is particularly concentrated in the region of the Golgi complex. Highest expression is found in brain, with lower levels detected in lung and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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