Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

View additional formats

• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Donkey/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Donkey/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Donkey/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Donkey/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Donkey/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Donkey/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Donkey/)
• [DyLight 755](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Donkey/)
• [DyLight 800](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• [Rhodamine](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_228234

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse,rat and sheep serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31568 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31568 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse,rat and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 8.3 µg/mg; >3 moles FITC per mole IgG. (lot-dependent).

Reconstitute with 0.4 mL of distilled water (1.5 mg/mL after restoration).

Country of Origin: USA

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.