Thermo Fisher Scientific Rabbit anti-Mouse IgM Secondary Antibody, FITC

Thermo Fisher Scientific Rabbit anti-Mouse IgM Secondary Antibody, FITC

Applications and Tested Dilution

Product Specifications

Additional Formats

• AP conjugate
• Biotin conjugate
• HRP conjugate
• Custom conjugation available upon request

Product Specific Information

• Concentration may vary slightly from lot to lot; refer to lot-specific datasheet for details.
• Product #31557 has been validated for Western blot, IF, ICC, IHC, IP, and FACS applications.
• Reacts specifically with the heavy chain of mouse IgM; does not react with normal mouse IgG or serum proteins.
• May cross-react with IgM from other species.
• Store protected from light at 4°C; for extended storage, add equal volume of glycerol (final 50%) and store at -20°C.
• Fluorescein Amax = 492 nm; Emax = 520 nm.
• Fluorophore/Protein ratio: 8.2 µg/mg (~3 moles FITC per mole IgG, lot-dependent).
• Rehydrate with 1.2 mL dH₂O; centrifuge if not clear. Prepare working dilution on day of use.
• Country of Origin: USA.

Target Information

Anti-Mouse secondary antibodies are affinity-purified and exhibit well-characterized specificity for mouse immunoglobulins. They are widely used for detection, sorting, and purification of target molecules.
Secondary antibodies enhance versatility and sensitivity, allowing use with various detection systems such as HRP, AP, or fluorescence.
Produced by immunizing host animals with pooled mouse immunoglobulins, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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