Thermo Fisher Scientific Rabbit anti-Mouse IgM Secondary Antibody, FITC

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

1:50 - 1:200

Flow Cytometry (Flow)

1:50 - 1:200

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

View additional formats

• [AP](/antibody/secondary/product-name/Mouse IgM/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgM/conjugates/Biotin/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Mouse IgM/conjugates/HRP/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_228443

Target

IgM

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31557 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31557 reacts with the heavy chain portion of mouse IgM, but does not react against normal mouse IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 8.2 µg/mg; ~ 3 moles FITC per mole IgG (lot-dependent).

Rehydrate with 1.2 mL dH2O and centrifuge if not clear. Prepare working dilution on day of use.

Country of Origin: USA

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.