Thermo Fisher Scientific Periostin (POSTN) Monoclonal Antibody (POSTN, 3503)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Periostin (PN), also known as osteoblast-specific factor 2 (OSF-2), is a disulfide-linked, secreted protein originally identified as an osteoblast-specific factor. It binds heparin and serves as a ligand for αVβ3 and αVβ5 integrins.
In preosteoblasts, Periostin functions as a cell adhesion molecule, promoting osteoblast recruitment, spreading, and attachment. It is mainly expressed in the lower gastrointestinal tract, aorta, stomach, placenta, uterus, and breast tissues, but is upregulated in epithelial ovarian tumors and overexpressed in breast cancer.
Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1 (TGF-β1). The protein contains a signal sequence, a cysteine-rich domain, four fasciclin-like repeats, and a C-terminal domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.