Norgen Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits

Norgen Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits

SKU: TM31550

Features and Benefits

• Detection kits for HPV (High and Low Risk)
• Available in TaqMan format for analysis

More than 70 types of human papillomavirus (HPV) have been identified and are generally classified as high-risk or low-risk depending on their association with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3).
HPV viruses are predominantly sexually transmitted, and high-risk HPV types are a major risk factor for cervical cancer development.
Low-risk types such as HPV 6 and 11 are associated with genital warts, while other low-risk types are not linked to warts or cervical cancer.

HPV cannot be cultured in vitro, and immunological tests are inadequate for detection. Biopsies can be analyzed by nucleic acid hybridization to detect HPV DNA directly.
HPV 16 and 18 are high-risk cancer-associated types, while HPV 31, 33, and 35 have intermediate risk.
Norgen’s HPV (High and Low Risk) TaqMan PCR Detection Kits are suitable for detecting HPV 33 and HPV 58.

Available Formats

HPV (High and Low Risk) TaqMan PCR Kit (100 reactions)

• Ready-to-use format with Master Mix and PCR control
• Specific Primer and Probe mix for HPV detection
• Includes Positive and Negative Controls

HPV (High and Low Risk) TaqMan PCR Probe/Primer Set and Controls (100 reactions)

• Specific Primer/Probe mix and Positive Control
• Includes Nuclease-free water
• Compatible with Norgen’s PCR Master Mix (#28007) or user-supplied mix

For research use only. Not intended for in vitro diagnostics.

Supporting Data

Figure 1. TaqMan PCR Positive Result

Both FAM and HEX channel signals rise above baseline, indicating successful PCR amplification.
그래프 정보 (OCR 추출):

• 상단 그래프: Amplification (FAM channel), Y축 RFU, X축 Cycles
• 하단 그래프: Amplification (HEX channel), Y축 RFU, X축 Cycles
  두 그래프 모두 증폭 곡선을 나타내며, FAM은 파란색, HEX는 초록색으로 표시됩니다.

Figure 2. TaqMan PCR Negative Result

No target DNA detected in FAM channel; HEX channel amplification confirms successful PCR.
그래프 정보 (OCR 추출):

• 상단: FAM channel, RFU(-100~400), Cycles(10~40)
• 하단: HEX channel, RFU(-100~800), Cycles(10~40)

Figure 3. TaqMan PCR Inhibition Result

No signal from FAM or HEX channels detected. Sample preparation should be repeated using recommended DNA purification kit.
그래프 정보 (OCR 추출):

• 상단: FAM channel, X축 Cycles(0~40), Y축 RFU(-100~900)
• 하단: HEX channel, 동일한 축 구조

Figure 4. End-Point PCR Result

A 1X TAE, 1.4% agarose gel shows amplification of HPV (High and Low Risk) at various concentrations.
OCR 추출 텍스트:

+   -   +   -   M
bp
2000
1500
1000
750
500
300
150
50
Target (280 bp)
PCR con

Storage Conditions and Product Stability

• Store all components at -20°C upon arrival.
• Avoid repeated freeze-thaw cycles (>2x) of Master Mix and Positive Control.
• Shelf life: 2 years from production without performance loss.
• For intermittent use, aliquot reagents before freezing.

Kit Components

Documentation

• (TM31550) HPV (High and Low Risk) TaqMan PCR Kit - Protocol (100 rxns)
• (TM31510) HPV High and Low Risk TaqMan Probe/Primer and Control Set - Protocol (100 rxns)

Related Product

Urine DNA Isolation Kit (Slurry Format)

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