Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 9 publications 9 publications

Neutralization (Neu)

-

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

-

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TRFK4

Immunogen

Recombinant mouse IL-5

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223534

Product Specific Information

MM550D targets IL-5 in ELISA, IA, and WB applications and shows reactivity with mouse samples.

The MM550D immunogen is recombinant mouse IL-5.

MM550D detects IL-5 which has a predicted molecular weight of approximately 13 kDa.

The MM550D mouse IL5 antibody (clone TRFK4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM550CB (biotinylated conjugate of clone TRFK5). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM550D (clone TRFK4) and biotinylated antibody MM550CB (clone TRFK5) have successfully been used in combination with recombinant mouse IL5 protein SMIL5 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product is a Low Endotoxin formulation.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.