Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4)

Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin conjugate (AMC0959)
• Request custom conjugation

Product Specific Information

MM550D targets IL-5 in ELISA, IA, and WB applications and shows reactivity with mouse samples.
The MM550D immunogen is recombinant mouse IL-5.
MM550D detects IL-5, which has a predicted molecular weight of approximately 13 kDa.

The MM550D mouse IL-5 antibody (clone TRFK4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM550CB (biotinylated conjugate of clone TRFK5).
Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125–0.25 µg/mL for detection.

Antibody MM550D (clone TRFK4) and biotinylated antibody MM550CB (clone TRFK5) have successfully been used in combination with recombinant mouse IL-5 protein SMIL5 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product is a Low Endotoxin formulation.
It has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration ≤10 EU/mg.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils.
It regulates eosinopoiesis, eosinophil maturation, and activation.
Elevated IL-5 production is associated with asthma and hypereosinophilic syndromes.

The IL-5 receptor is a heterodimer whose beta subunit is shared with receptors for IL-3 and GM-CSF (CSF2).
IL-5, IL-4, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q31, regulated by long-range elements spanning ~120 kb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.