Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK5), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 14 publications 14 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

TRFK5

Immunogen

Recombinant mouse IL-5

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223606

Product Specific Information

MM550CB targets IL-5 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM550CB immunogen is recombinant mouse IL-5.

MM550CB detects IL-5 which has a predicted molecular weight of approximately 13 kDa.

The MM550CB mouse IL5 antibody (biotinylated conjugate of clone TRFK5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM550D (clone TRFK4). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM550CB (clone TRFK5) and antibody MM550D (clone TRFK4) have successfully been used in combination with recombinant mouse IL5 protein SMIL5 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.