Thermo Fisher Scientific Cyclin B3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin B3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553982

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 158 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Cell proliferation is controlled at specific stages of the cell cycle by distinct protein kinase complexes. These complexes consist of a catalytic subunit associating with a specific regulatory subunit to form the active kinase. The cyclins, which include cyclin A, B, C, D, E, F, G, H, I, K, L, T and their related proteins, including Dbf4, comprise the regulatory subunits of these kinase complexes. The controlled activation of the kinase complexes at various intervals of the cell cycle is regulated by the availability of the cyclins to the catalytic subunit. Unlike the catalytic subunit, which is expressed continually, the expression and stability of the regulatory subunit fluctuates depending on the stage of the cell cycle, thereby regulating kinase activity. Cyclin B3, also known as CCNB3 or CYCB3, is a 1,395 amino acid nuclear protein that belongs to the cyclin family of regulatory proteins. Expressed in testis with lower expression in a variety of other tissues, cyclin B3 is thought to be required for early meiotic prophase I, playing an important role in the meiotic cell cycle. Three isoforms of cyclin B3 exist due to alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.