Thermo Fisher Scientific CD133 Polyclonal Antibody
인간 CD133 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot과 IHC(Paraffin)에서 검증됨. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 줄기세포 및 암 관련 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PROM1 (CD133, Prominin-1, UniProt ID: O43490-1, amino acids 618–747) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2790333 |
Product Specific Information
Immunogen sequence:
LQDFAACGID RMNYDSYLAQ TGKSPAGVNL LSFAYDLEAK ANSLPPGNLR NSLKRDAQTI KTIHQQRVLP IEQSLSTLYQ SVKILQRTGN GLLERVTRIL ASLDFAQNFI TNNTSSVIIE ETKKYGRTII
Target Information
CD133 (AC133) antigen is a 97 kDa glycoprotein with 5 transmembrane domains, appearing as a 120 kDa band on protein gels due to glycosylation. It is a cell surface marker expressed by immature hematopoietic stem cells, but not by mature blood cells. CD133 is also a marker for other stem and progenitor cells, including neural and embryonic stem cells. Expression has been observed in certain cancers, such as leukemias and brain tumors, and at low levels in kidney, pancreas, placenta, and fetal liver. Mutations in the CD133 gene are associated with retinitis pigmentosa and Stargardt disease. Multiple transcript variants exist due to alternative promoter usage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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