Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Functional Grade, eBioscience
인간 CD8a 분자를 인식하는 OKT8 단클론 항체로, T 세포 및 NK 세포의 활성화 연구에 적합합니다. Flow cytometry 등 다양한 응용 가능하며, Functional Grade로 제조되어 세포 기능 분석에 사용됩니다. 고순도(>90%) 및 저 엔도톡신 수준으로 연구용에 최적화된 제품입니다.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Functional Grade, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | 22 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| Functional Assay (Functional) | Assay-Dependent | 1 publication |
| Control (Ctrl) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Cynomolgus monkey, Human, Non-human primate |
| Host/Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2865609 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin, eFluor 450, FITC
- NovaFluor Blue/Red/Violet/Yellow series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a CD8αβ heterodimer or CD8αα homodimer. CD8a is found on most thymocytes and subsets of mature T and NK cells. It binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a key role in T-cell development and activation.
Preliminary testing indicates that OKT8, RPA-T8, and HIT8a clones do not compete for binding to human peripheral blood leukocytes, suggesting distinct epitopes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells at ≤0.25 µg/test (100 µL final volume). Optimize titration for best assay performance.
Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction, T cell differentiation, and activation. It functions as a co-receptor for MHC class I molecules, critical for cytotoxic T lymphocyte (CTL) activation and pathogen/tumor clearance. CD8 is expressed on T cells, NK cells, monocytes, and dendritic cells, forming disulfide-linked dimers. Activation is enhanced by cytokines such as IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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