Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Functional Grade, eBioscience
Flow cytometry 및 T 세포 활성화 연구용 CD8a 단클론 항체로, 마우스 CD8 분자를 인식합니다. 1 mg/mL 농도의 액상 형태이며, 4°C에서 보관합니다. Functional Grade 등급으로 다양한 형광 및 효소 표지 형식 제공.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Functional Grade, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 24 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 7 |
| Flow Cytometry (Flow) | 0.25 µg/test | 181 |
| ELISA | - | 2 |
| Immunoprecipitation (IP) | - | 1 |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | 4 |
Product Specifications
| Property | Description |
|---|---|
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865606 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395–805)
- Brilliant Violet (421–786)
- eFluor (450, 506, 615, 660)
- FITC
- NovaFluor (Blue, Red, Violet, Yellow series)
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright (436–780)
- Custom conjugation available upon request
Product Specific Information
Description:
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either CD8αβ heterodimer or CD8αα homodimer. CD8 binds MHC class I and, via p56lck association, plays a key role in T cell development and activation.
Applications Reported:
Useful for flow cytometric analysis, T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding.
Applications Tested:
Validated by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤0.25 µg/test (100 µL final volume, 10^5–10^8 cells/test). Optimize titration for best assay performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90% (SDS-PAGE)
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)
Aggregation:
<10% (HPLC)
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein and co-receptor for MHC class I molecules. It is primarily expressed on T cells as CD8αβ heterodimers and on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8αα homodimers. CD8 engagement with MHC-I/peptide complexes recruits Lck kinase, leading to CTL activation and immune response. Differentiation of CD8+ T cells into cytotoxic T lymphocytes is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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