Thermo Fisher Scientific CD200 Monoclonal Antibody (OX104), Biotin, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.06 µg/test
  View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody OX104 recognizes human CD200 (OX2), a member of the immunoglobulin superfamily with two Ig domains, a transmembrane, and a cytoplasmic domain. CD200 is expressed on resting and activated B cells, subsets of T cells, keratinocytes, nerve cells, follicular dendritic cells, and ovarian cells. Interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation, and reduced TNFα and IL-13 secretion, leading to attenuation of lymphocyte activation. CD200 expression on trophoblasts suggests a role in maternal tolerance.

Applications Reported:
This OX104 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤ 0.06 µg per test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Target Information

CD200 is a type-1 membrane glycoprotein with two immunoglobulin domains, belonging to the immunoglobulin superfamily. It interacts with CD200R on myeloid cells to inhibit macrophage activation. Homologues exist in herpesviruses and poxviruses. CD200 regulates myeloid cell activity and delivers inhibitory signals to macrophages. Multiple alternatively spliced variants encode different isoforms. CD200 dysfunction is associated with Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.