Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PerCP-eFluor 710, eBioscience

Applications

Flow Cytometry (Flow)

• Tested dilution: 0.5 µg/test
• View 9 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 480
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR-bearing T cells, a subset of γ/δ-TCR-bearing T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein structurally similar to CD4. It can be proteolytically cleaved into a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The 54 kDa portion may remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4, playing a role in negative regulation of T-cell activation and homeostatic proliferation. It is also expressed by CD4⁺CD25⁺ regulatory T cells and may contribute to their suppressive function.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal dilution should be determined experimentally.

Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (Blue laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable alternative)

PerCP-eFluor® 710 conjugates are stable when exposed to freshly prepared 2% formaldehyde overnight at 4°C. Evaluate alternative fixation protocols as needed.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily homologous to CD4. It binds MHC class II with higher affinity than CD4 and is involved in negative regulation of T-cell activation and proliferation.
LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells. CD8⁺ T cells express higher levels than CD4⁺ T cells. Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1 cells) in humans and mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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