Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 615, eBioscience

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG

Class

Monoclonal

Type

Secondary Antibody

Clone

M1-14D12

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

View additional formats

• [APC ](/antibody/secondary/product-name/Mouse IgG1/conjugates/APC/host/Rat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG1/conjugates/Biotin/host/Rat/)
• [eFluor 450](/antibody/secondary/product-name/Mouse IgG1/conjugates/eFluor 450/host/Rat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG1/conjugates/eFluor 570/host/Rat/)
• [eFluor 660](/antibody/secondary/product-name/Mouse IgG1/conjugates/eFluor 660/host/Rat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG1/conjugates/FITC/host/Rat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG1/conjugates/HRP/host/Rat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG1/conjugates/PE/host/Rat/)
• [PE-Cyanine7](/antibody/secondary/product-name/Mouse IgG1/conjugates/PE-Cyanine7/host/Rat/)
• [PE-eFluor 610](/antibody/secondary/product-name/Mouse IgG1/conjugates/PE-eFluor 610/host/Rat/)
• [PerCP-eFluor 710](/antibody/secondary/product-name/Mouse IgG1/conjugates/PerCP-eFluor 710/host/Rat/)
• [Super Bright 436](/antibody/secondary/product-name/Mouse IgG1/conjugates/Super Bright 436/host/Rat/)
• [Super Bright 600](/antibody/secondary/product-name/Mouse IgG1/conjugates/Super Bright 600/host/Rat/)
• [Super Bright 780](/antibody/secondary/product-name/Mouse IgG1/conjugates/Super Bright 780/host/Rat/)
• Request custom conjugation

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042585

Target

IgG1

Antibody Form

Whole Antibody

Product Specific Information

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.

Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.

Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.