Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 615, eBioscience

Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 615, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• APC
• Biotin
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• HRP
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a secondary reagent in flow cytometry and microscopy. It does not recognize other mouse isotypes nor cross-react with rat IgG1 or any rat isotype antibodies.

Applications Reported: Immunohistochemical staining and immunocytochemistry.
Applications Tested: Tested by immunocytochemistry and immunohistochemistry using mouse IgG1 primary antibodies at ≤5 µg/mL. Antibody titration recommended for optimal performance. Not validated for flow cytometry.

Filter Recommendation: Use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor® 594 filters are acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of targets and enable versatile detection systems (HRP, AP, fluorescence). Secondary antibodies enhance sensitivity via signal amplification as multiple secondary antibodies can bind to a single primary antibody. Typically generated by immunizing host animals with pooled mouse immunoglobulins, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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