Thermo Fisher Scientific MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1) Monoclonal Antibody (2F6)
MAP3K1 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체(2F6)입니다. Western blot 및 면역조직화학(IHC) 분석에 적합하며, 인간 시료에 반응합니다. Protein A/G 정제, 액상 형태, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F6 |
| Immunogen | Partial recombinant MAP3K1 (aa1077–1176): SKNSMTLDL NSSSKCDDSF GCSSNSSNAV IPSDETVFTP VEEKCRLDVN TELNSSIEDL LEASMPSSDT TVTFKSEVAV LSPEKAENDD TYKDDVNHNQK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. These kinases are highly conserved across species including yeast, Drosophila, and mammals. MEKK1 can phosphorylate and activate MAPKK1 and MAPKK2 (MEK1/MEK2), leading to MAP kinase activation, and is also a strong activator of the JNK cascade. The protein contains a RING-type zinc finger and a SWIM-type zinc finger.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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