Thermo Fisher Scientific WIBG Monoclonal Antibody (OTI2C7), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

WIBG is a key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs. It serves as a positional landmark for the intron-exon structure of genes and directs post-transcriptional processes such as mRNA export, nonsense-mediated mRNA decay (NMD), and translation.
Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit prevents translation-independent disassembly, ensuring activity is restricted to translated mRNAs.
WIBG interferes with NMD and enhances translation of spliced mRNAs, likely by antagonizing EJC functions. RNA-binding ability has been reported inconsistently in literature (PubMed:14968132; PubMed:19410547).
[Source: UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.