Thermo Fisher Scientific ZO-1 Recombinant Mouse Monoclonal Antibody (ZO1-1A12)
ZO-1 단백질을 인식하는 재조합 마우스 단일클론 항체로, Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 세포 간 밀착연접 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:2,000 |
| Flow Cytometry (Flow) | 0.063 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ZO1-1A12 |
| Immunogen | Human recombinant ZO-1 fusion protein encompassing amino acids 334–634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074173 |
Available Conjugated Formats
- Alexa Fluor Plus 488 (Catalog 740002MP488)
- Alexa Fluor Plus 555 (Catalog 740002MP555)
- Alexa Fluor Plus 647 (Catalog 740002MP647)
- Custom conjugation available (Request Form)
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Specific genes encoding the desired antibody are cloned into an expression vector and expressed in vitro.
Advantages include higher specificity and consistent performance between lots.
It is recommended to titrate the antibody for optimal performance in each application.
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the tight junction.
ZO-1 expression is reduced in many breast cancer lines.
ZO-1 is located on the cytoplasmic surface of intercellular tight junctions and may be involved in signal transduction.
Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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