Thermo Fisher Scientific Cdc25A Polyclonal Antibody
Rabbit polyclonal antibody against human Cdc25A. Validated for WB and IHC-P applications. High purity (>95%) and antigen affinity purified. Supplied as liquid in PBS with 50% glycerol. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to Human Cdc25A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803675 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
CDC25A is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C, which function at different phases of the cell cycle. CDC25A is required for progression through G1 and S phases, activating cyclin-dependent kinase CDC2 by removing two phosphate groups. It is specifically degraded in response to DNA damage, preventing cells with chromosomal abnormalities from dividing. CDC25A is considered an oncogene, though its precise role in oncogenesis has not been fully demonstrated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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