Thermo Fisher Scientific Kinesin 5B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:6,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

Immunoprecipitation (IP)

5 µg

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C I(376) D E Q F D K E K A N L E A F T V D K D I(396) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Kinesin 5B,uniProtId:P33176-1,ncbiNodeId:9606,antigenRange:376-396,antigenLength:963,antigenImageFileName:PA1-643_Kinesin_5B_P33176-1_Rabbit.svg,antigenImageFileNamePDP:PA1-643_Kinesin_5B_P33176-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2234330

Product Specific Information

PA1-643 detects kinesin 5B from mouse and human samples. This antibody is specific for kinesin 5B and does not detect other kinesin isotypes.

PA1-643 has been successfully used in Western blot and immunofluorescent procedures. By Western blot, this antibody detects an ~110 kDa protein representing kinesin 5B protein.

The PA1-643 immunizing peptide corresponds to amino acid residues 376-396 from human kinesin 5B. This sequence is 95% and 90% conserved for mouse and rat, respectively. This peptide (Cat. # PEP-198) is available for use in neutralization and control experiments.

Target Information

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.