Thermo Fisher Scientific CRM1 Recombinant Rabbit Monoclonal Antibody (9F2)
Recombinant rabbit monoclonal antibody recognizing human CRM1 (Exportin-1). Validated for IHC (P) and ELISA. High purity via affinity chromatography. Supplied in PBS with 50% glycerol for stable long-term storage. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9F2 |
| Immunogen | A synthesized peptide derived from human CRM1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3092818 |
Target Information
Exportin-1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of the nuclear complex into the cytoplasm, disassembly and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of nuclear export is conferred by the asymmetric distribution of GTP- and GDP-bound forms of Ran between cytoplasm and nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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