Thermo Fisher Scientific Histone H2B Polyclonal Antibody
Rabbit polyclonal antibody targeting Human Histone H2B N-terminus. Validated for WB, IHC, and ICC applications. High purity (>95%) and antigen affinity purified. Supplied in PBS with glycerol; ideal for chromatin and transcription research.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids at the N-terminus of Human Histone H2B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803104 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of the four core histones (H2A, H2B, H3, and H4). These nucleosomes compact DNA into chromatin, regulating accessibility for transcription, replication, and repair. Histone H2B plays a central role in transcription regulation, DNA repair, and chromosomal stability, and exhibits broad antibacterial activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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