Thermo Fisher Scientific CD47/IAP (Integrin Associated Protein) Recombinant Rabbit Monoclonal Antibody (CD47, 6362R)

Applications

Tested Applications and Dilutions

Product Specifications

Product Specific Information

• IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.

Target Information

CD47 (Integrin-Associated Protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, including activation of heterotrimeric G proteins.

Functionally, CD47 participates in increasing intracellular calcium concentration upon cell adhesion to the extracellular matrix. It also serves as a receptor for the C-terminal cell-binding domain of thrombospondin, playing roles in membrane transport and signal transduction.

CD47 is broadly distributed across tissues but reduced on Rh erythrocytes. It acts as a ligand for CD172a (signal regulatory protein alpha, SIRPα), an immune inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. The CD47–CD172a system influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, particularly in synapse-rich regions of the brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.