Thermo Fisher Scientific MSH6 Monoclonal Antibody (SP93)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:250

-

Flow Cytometry (Flow)

1:20

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP93

Immunogen

Synthetic peptide corresponding to internal region of human MSH6 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537899

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is rectal carcinoma.

Target Information

The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for the detection of DNA damage, and may promote apoptosis in normal cells, thus protecting mammals from ultraviolet-induced malignant transformation and hence the prevention of skin cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.