Thermo Fisher Scientific Cre recombinase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

-

Immunohistochemistry (IHC)

-

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Product Specifications

Species Reactivity

Virus

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to the center region of Cre recombinase

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549717

Product Specific Information

Recommended positive controls: 293T transfected recombinant cre protein, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.