Thermo Fisher Scientific USP9X Monoclonal Antibody (OTI2B4), TrueMAB
USP9X 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 적합합니다. 인간 유래 단백질 조각을 면역원으로 사용하였으며, 동결건조 형태로 제공됩니다. PBS 기반 버퍼에 보존제가 포함되어 있지 않습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2246–2570 of human USP9X produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20 °C, avoid freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product #89882).
Target Information
USP9X is an ubiquitin-specific protease that removes and recycles ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9X plays a critical role in tight junction biogenesis by regulating levels of the Arf6 exchange factor (EFA6), which facilitates tight junction formation during the establishment of cell polarity.
Located on the X-chromosome, USP9X escapes X-inactivation, and its defects have been associated with gonadal degeneration in Turner syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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