Thermo Fisher Scientific SMAD2 Monoclonal Antibody (OTI4G7), TrueMAB
SMAD2 단백질을 인식하는 Mouse Monoclonal 항체로, Western Blot, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 세포 증식, 분화, 아폽토시스 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G7 |
| Immunogen | Full length human recombinant protein of human SMAD2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SMAD2 (MADH2, MAD2) regulates various cellular processes including proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors via the SMAD anchor for receptor activation, a central event in TGF-beta signaling.
Phosphorylation of threonine 8 by ERK1 enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. Upon TGF-beta signaling, SMAD2 is phosphorylated, dissociates from SARA, and associates with SMAD4 for nuclear translocation. In the nucleus, SMAD2 binds target promoters and forms transcription repressor complexes. It can also be phosphorylated by activin type 1 receptor kinase, mediating activin signaling.
Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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