Thermo Fisher Scientific CD14 Monoclonal Antibody (OTI12C8), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on the surface of mature monocytes, macrophages, and neutrophils.
It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms.
CD14 binds lipopolysaccharides (LPS) in a reaction catalyzed by lipopolysaccharide-binding protein (LBP). The soluble form (sCD14) helps neutralize serum LPS and can bind apoptotic cells, influencing allergic, inflammatory, and infectious processes.
Alternative splicing results in multiple transcript variants encoding the same CD14 isoform.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.