Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), APC, eBioscience

Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 725
• NovaFluor Blue 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. It is used as a phenotypic marker for TCR beta expressing T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes at ≤0.125 µg/test (100 µL final volume). Recommended to titrate for optimal performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response. Recognition of self-peptides is linked to autoimmune disease, while mutant self-peptides are associated with tumors. Engagement by bacterial superantigens can cause toxic shock syndrome. Autoantibodies to TCR V beta segments have been found in rheumatoid arthritis and lupus patients, potentially modulating inflammatory responses.

Most human T cells express TCR alpha-beta with CD4 or CD8 (single positive). A small subset lacks both (double negative), which can increase in autoimmune or immunodeficiency disorders. Gamma-delta T cells are found in epithelial tissues and exhibit distinct antigen recognition and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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