Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Available Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes.
HLA-A3 is expressed by 15–25% of cancer patients and binds the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use 5 µL (0.6 µg) per test (100 µL final volume).

Recommended Cell Number: 10⁵–10⁸ cells/test

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample regardless of the number of NovaFluors.
• For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This molecule is a heterodimer of a heavy chain and beta-2 microglobulin. Class I molecules present peptides derived from the endoplasmic reticulum lumen and are expressed in nearly all cells.
The heavy chain (~45 kDa) gene contains 8 exons:

• Exon 1: leader peptide
• Exons 2–3: alpha1 and alpha2 domains (peptide binding)
• Exon 4: alpha3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2–3 determine peptide binding specificity. Typing is routinely performed for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification via LC-MS/MS.

For Research Use Only.
Not for diagnostic use or resale without authorization.

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