Thermo Fisher Scientific CD33 Monoclonal Antibody (9A11-CD33), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 0.25 µg/test
  • View 2 publications

Product Specifications

Available Formats:

• Alexa Fluor 647
• APC
• PE
• Super Bright 436
• Super Bright 702
  Request custom conjugation

Product Specific Information

This monoclonal antibody (clone 9A11-CD33) reacts with mouse CD33, also known as Siglec3 or gp67.
CD33 belongs to the sialic acid-binding immunoglobulin lectins (Siglec) family, which binds to sialic acid-containing glycans on cell surface glycoproteins and glycolipids.

• Mouse CD33 binds to short O-linked glycans of certain mucins and sialyl-Tn epitopes.
• Expressed by mature granulocytes, immature myeloid cells, and microglia.
• Unlike human CD33, mouse CD33 lacks a cytoplasmic ITIM domain and exists as two splice variants with different cytoplasmic tails.
• In microglia, CD33 inhibits phagocytosis of toxic amyloid beta.

Clone 9A11 has been used to characterize mouse CD33 surface expression and function.
View Article (PubMed: 31925286)

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤ 0.25 µg/test (in 100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Antibody titration is recommended for optimal performance.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Store up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD33 is a transmembrane protein of the Siglec family containing ITIM motifs that recruit SHP-1 and SHP-2 phosphatases.
These motifs mediate signaling and receptor removal from the cell surface via ubiquitin pathways.

• Expressed on myelomonocytic lineage cells
• Binds sialic acid residues in N- and O-glycans
• Therapeutic target for acute myeloid leukemia (AML)
• Expressed on granulocyte and macrophage precursors, but not pluripotent stem cells
• Useful marker for peripheral monocytes and distinguishing myelogenous from lymphoid or erythroid leukemias
• CD33 dysfunction is associated with gallbladder lymphoma and extracutaneous mastocytoma

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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