Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Suggested positive control: antigen standard for TUBB (transient overexpression lysate), HeLa whole cell extract.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the function of this modification is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, which inhibits tubulin incorporation into microtubules.

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin dimers (alpha and beta subunits, each ~55 kDa). They play key roles in chromosome segregation, intracellular transport, ciliary and flagellar motion, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control in protein detection assays such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.