Thermo Fisher Scientific Decorin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Horse, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 192 of Human Decorin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Decorin,uniProtId:P07585-1,ncbiNodeId:9606,antigenRange:1-192,antigenLength:359,antigenImageFileName:PA5-27370_Decorin_P07585-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27370_Decorin_P07585-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 40% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544846

Product Specific Information

Recommended positive controls: A549, H1299.

Predicted reactivity: Mouse (82%), Rat (80%), Dog (91%), Pig (88%), Rabbit (90%), Sheep (86%), Chimpanzee (100%), Bovine (87%), Guinea pig (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Decorin is a member of the small, leucine-rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF-b. It is believed that decorin also plays a role in the cell cycle of chondrocytes. The decorin neoepitope fragment has a sequence of PKTLQE, and is generated by MMP-3 or ADAMTS-4 cleavage at Glu154-Leu155 of decorin core protein.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.