Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular staining of normal human lysed whole blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended ≤1.0 µg per test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test).
Optimize antibody titration for best performance.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is excited by a 405 nm violet laser. Ensure cytometer compatibility.

Staining Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (#00-8222-49) or 1-Step Fix/Lyse Solution (#00-5333-54).
BV650 is not compatible with methanol-based fixation.

Excitation / Emission: 407 nm / 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine into urea and L-ornithine, the final step in the urea cycle.
The resulting polyamines support cell proliferation and detoxification.
Arginase 1 modulates nitric oxide production by depleting L-arginine, the substrate for NO synthase.

In both human and mouse, Arginase 1 is expressed in:

• Liver
• Neutrophils
• Myeloid-derived suppressor cells (MDSCs)
• Neural stem cells

Human expression is observed in blood neutrophils (not in CCR3⁺ granulocytes).
In mice, Arginase 1 is a hallmark of alternatively activated macrophages (M2a).
It may also be expressed in tumor-infiltrating myeloid cells and hepatocellular carcinomas.
Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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