Thermo Fisher Scientific Granzyme B Monoclonal Antibody (GB11), PE-Texas Red

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

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Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GB11

Immunogen

Human Granzyme B.

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

View additional formats

• APC
• PE
• PE-Cyanine5.5
• Request custom conjugation

Excitation/Emission Max

567/615 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536540

Product Specific Information

According to the literature this antibody recognizes the serine protease, granzyme B1. This enzyme is involved in apoptotic cell death. Granzyme B, as well as other enzymes contained in lytic granules, is released primarily by cytotoxic T lymphocytes (CTLs). In addition to immunostaining for flow cytometry, the GB11 mAb has been shown to be useful as an ELISA capture antibody.

Target Information

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.