Thermo Fisher Scientific alpha Internexin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:20,000

-

Immunohistochemistry (IHC)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Bovine, Horse, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified, recombinant rat alpha-internexin, expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2127487

Product Specific Information

In Western blot, a prominent band at ~66 kDa is observed, as well as smaller lower bands which are apparently degradation products. A minor band at ~150 kDa is also seen, resulting from dimerization of alpha-internexin.

Target Information

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.