Thermo Fisher Scientific Carbonic Anhydrase IX Polyclonal Antibody
Carbonic Anhydrase IX 단백질을 인식하는 Thermo Fisher의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용 가능. 인간 및 마우스 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로, 안정적인 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.97 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2735571 |
Product Specific Information
- Positive Control: HepG2 (untreated), HepG2 (200 µM CoCl₂ treatment for 24 hr)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxia via HIF-1α, due to its stability and membrane localization.
Carbonic anhydrases regulate pH by controlling hydrogen ion (H⁺) flux, and inhibition of CA IX increases cell death under hypoxic conditions.
Therefore, CA IX serves as a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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