Thermo Fisher Scientific Click-iT RNA Alexa Fluor 594 Imaging Kit

Invitrogen™ Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit

The Click-iT® RNA Alexa Fluor® 594 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84).
Detection of newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage, or drug treatments is an important aspect of toxicological profiling.

This kit uses an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and click chemistry to detect newly synthesized RNA without radioactivity or antibodies in a simple two-step procedure:

1. The alkyne-containing nucleoside is fed to cells or animals and incorporated into nascent RNA.
2. Detection occurs via chemoselective ligation (“click” reaction) between an azide and an alkyne, where modified RNA is detected with an azide-containing dye.

The small size of the Click-iT® detection molecule allows efficient penetration of complex samples and enables multiplex analyses with other probes, including antibodies for RNA-interactive proteins, providing deeper biological insights.

사양 (Specifications)

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