Thermo Fisher Scientific SDE2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SDE2. Recombinant protein control fragment (Product #RP-94307). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SDE2,uniProtId:Q6IQ49-1,ncbiNodeId:9606,antigenRange:118-216,antigenLength:451,antigenImageFileName:PA5-55868_SDE2_Q6IQ49-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55868_SDE2_Q6IQ49-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647048

Product Specific Information

Immunogen sequence: MAEWVKQQAE REAEKEQKRL ERLQRKLVEP KHCFTSPDYQ QQCHEMAERL EDSVLKGMQA ASSKMVSAEI SENRKRQWPT KSQTDRGASA GKRRCFWLG

Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 82%.

Target Information

Involved in both DNA replication and cell cycle control (PubMed:27906959). Unprocessed SDE2 interacts with PCNA via its PIP-box. The interaction with PCNA prevents monoubiquitination of the latter thereby inhibiting translesion DNA synthesis. The binding of SDE2 to PCNA also leads to processing of SDE2 by an unidentified deubiquitinating enzyme, cleaving off the N-terminal ubiquitin-like domain. The resulting mature SDE2 is degraded by the DCX(DTL) complex in a cell cycle- and DNA damage dependent manner (PubMed:27906959). Binding of SDE2 to PCNA is necessary to counteract damage due to ultraviolet light induced replication stress. The complete degradation of SDE2 is necessary to allow S-phase progression (PubMed:27906959). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.