Thermo Fisher Scientific H4K20me3 Monoclonal Antibody (JSM20), ChIP-Verified
H4K20me3 단백질을 특이적으로 인식하는 단클론 항체로, ChIP 및 Western blot에 검증됨. 인간, 마우스, 랫, 영장류 등 다양한 종에 반응. 고순도 정제 및 안정한 글리세롤 보존 용액 제공. 연구용으로 염색질 구조 및 히스톤 변형 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ChIP assay (ChIP) | 1–5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JSM20 |
| Immunogen | A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is tri-methylated. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 248 µg/mL |
| Purification | Purified |
| Storage buffer | 10 mM HEPES buffered saline, pH 7.5, with 150 mM NaCl, 50% glycerol, 100 µg/mL BSA |
| Contains | <0.02% sodium azide |
| Storage conditions | –20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2884049 |
Product Specific Information
Species predicted to react based on 100% sequence homology to Xenopus, Bovine, and Porcine.
Click here for Master Lot linking information.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher-order structures.
This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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