Thermo Fisher Scientific Fibronectin Monoclonal Antibody (A22)
소 우래 섬유연소 단백질(Fibronectin)을 특이적으로 검출하는 단클론 항체. Western blot, ELISA, IHC(F), IP 등 다양한 응용 가능. 인간 Fibronectin에는 반응하지 않으며, 다른 결합조직 단백질과 교차 반응 없음. 연구용으로만 사용.
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Thermo Fisher Scientific Fibronectin Monoclonal Antibody (A22)
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:100 | |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | - | |
| ELISA | 1:16,000 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A22 |
| Immunogen | Bovine corneal endothelial cells on beads |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.03 mg/mL (lot-dependent, 0.85–1.15 mg/mL) |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1074410 |
Product Specific Information
CSI 005-22-02 detects fibronectin from bovine samples.
This antibody does not detect human fibronectin.
No cross-reactivity observed with vitronectin, elastin, collagen, or laminin.
Validated for use in Western blotting, ELISA, Immunohistochemistry (frozen), and Immunoprecipitation.
Target Information
Fibronectin is a disulfide-bonded dimer (230–250 kDa) found in connective tissues and plasma.
In the extracellular matrix, it forms insoluble, cross-linked polymers.
Functions include cell adhesion, migration, wound healing, embryogenesis, coagulation, host defense, and metastasis.
Fibronectin is highly expressed in non-small cell lung carcinoma and contributes to tumorigenicity and chemotherapy resistance.
The gene exhibits alternative splicing, producing up to 20 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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