Thermo Fisher Scientific Collagen V (soluble) Monoclonal Antibody (1E2-E4/Col5)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

ELISA (ELISA)

1:50

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Marsupial, Sheep, Pig, Rabbit

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1E2-E4/Col5

Immunogen

Acid-digested pepsin soluble dog type V collagen.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073787

Product Specific Information

CSI 006-01-02 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 006-01-02 shows reactivity with human, dog, calf, sheep, pig and kangaroo in type V collagens by ELISA and immunoblotting. CSI 006-01-02 does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and immunoblotting.

CSI 006-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. CSI 006-01 has been used succesfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of foetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

COL5A1 encodes an alpha chain for one of the lowabundance fibrillar collagens. Fibrillar collagen molecules aretrimers that can be composed of one or more types of alpha chains.Type V collagen is found in tissues containing type I collagen andappears to regulate the assembly of heterotypic fibers composed ofboth type I and type V collagen. COL5A1 product is closelyrelated to type XI collagen and it is possible that the collagenchains of types V and XI constitute a single collagen type withtissue-specific chain combinations. Mutations in this gene areassociated with Ehlers-Danlos syndrome, types I and II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.