Thermo Fisher Scientific Peroxidase Polyclonal Antibody
Western blot, IHC, ELISA 등 다양한 면역분석에 사용 가능한 과산화효소(HRP) 폴리클로날 항체. 기니피그 유래, 동결건조 형태로 높은 민감도와 안정성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 – 1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000 – 1:5,000 |
| ELISA | 1:20,000 – 1:100,000 |
| Immunoprecipitation (IP) | 1:2,000 – 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Guinea pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peroxidase (Horseradish) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase (Horseradish). Cross-reactivity against peroxidase from other tissues and species may occur but has not been specifically determined.
Target Information
Horseradish peroxidase (HRP) is an enzyme derived from the root of the horseradish plant. It catalyzes the oxidation of a wide range of substrates in the presence of hydrogen peroxide. HRP is widely used as a detection enzyme in immunoassays such as ELISA and Western blot due to its high sensitivity and stability. It is also used as a tracer in enzyme-linked assays and as a labeling agent in fluorescence microscopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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