Thermo Fisher Scientific BECN1 Monoclonal Antibody (OTI3H8), TrueMAB
Beclin 1 단백질을 인식하는 Mouse Monoclonal Antibody로, WB, IHC, ICC, Flow 분석에 적합합니다. 인간 및 랫트 반응성을 가지며, Lyophilized 형태로 제공됩니다. 자가포식 연구 및 세포 내 단백질 분석용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H8 |
| Immunogen | Full length human recombinant protein of human BECN1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.
Different PI3K complex forms are believed to play roles in multiple membrane trafficking pathways:
- PI3KC3-C1 is involved in the initiation of autophagosomes.
- PI3KC3-C2 is involved in maturation of autophagosomes and endocytosis.
Beclin 1 also contributes to antiviral host defense.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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