Thermo Fisher Scientific BCAR1/p130CAS Polyclonal Antibody
BCAR1/p130CAS 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, IP 등에 사용 가능. 인간 및 생쥐 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 275 and 325 of human breast cancer anti-estrogen resistance 1 (Crk-associated substrate p130Cas) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signals via v-Crk and v-Src oncoproteins. It has a unique structure containing an SH3 domain followed by multiple YXXP motifs and a proline-rich region.
p130Cas is involved in biological processes such as:
- Cell adhesion and migration
- Growth factor stimulation
- Cytokine receptor engagement
- Bacterial infection
Physiological functions include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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