Thermo Fisher Scientific PCAF Polyclonal Antibody
Rabbit polyclonal antibody against human PCAF (KAT2B). Validated for WB, IP, and ChIP-Seq. Recognizes residues 782–832 of p300/CBP-associated factor. Supplied as 1 mg/mL liquid, stored at 4 °C. Predicted to cross-react with mouse.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 782 and 832 of human p300/CBP-associated factor (PCAF, UniProt ID: Q92831-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4 °C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) promoting transcriptional activation. It acetylates histones H3 and H4, as well as non-histone proteins such as ACLY, PLK4, and TBX5. PCAF inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. It also acts as a circadian transcriptional coactivator enhancing NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimer activity, and is involved in heart and limb development through TBX5 acetylation and regulation of its nucleocytoplasmic shuttling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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