Thermo Fisher Scientific SFTPA1 Monoclonal Antibody (6F10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:3000

-

ELISA (ELISA)

1:4,000

-

Immunoprecipitation (IP)

-

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Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6F10

Immunogen

Native SP-A purified from bronchioaveolar lavage from proteinosis patients.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_1087623

Product Specific Information

HYB 238-04-02 is specific for human pulmonary SP-A.

HYB 238-04-02 has been successfully used in ELISA, Immunohistochemistry and Western blot procedures. In Western blotting after SDS-PAGE, HYB 238-04-02 reacts with reduced forms of SP-A and weaker with non-reduced forms. In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04-02 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. HYB 238-04-02 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.

The HYB 238-04-02 immunogen native SP-A purified from bronchioaveolar lavage from proteinosis patients.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.