Thermo Fisher Scientific CD45 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues.

CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms due to alternative splicing, differing in their extracellular domains but sharing identical transmembrane and cytoplasmic domains. CD45RA is an isoform with restricted expression between lymphoid cell subtypes.

CD45 isoforms differ in their ability to translocate into glycosphingolipid-enriched membrane domains, and their expression depends on cell type and physiological state. CD45 is an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. It also promotes cell survival by modulating integrin-mediated signal transduction, DNA fragmentation during apoptosis, and various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.