Thermo Fisher Scientific LSD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:400

-

Flow Cytometry (Flow)

1:400

-

Immunoprecipitation (IP)

1:50

-

ChIP assay (ChIP)

5 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LSD1,uniProtId:O60341-1,ncbiNodeId:9606,antigenRange:1,antigenLength:852,antigenImageFileName:PA5-17361_LSD1_O60341-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17361_LSD1_O60341-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

80 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982296

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

LSD1 is a histone demethylase that specifically demethylates Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys-4 of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys-4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate Lys-9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.