Thermo Fisher Scientific Bcl-W Polyclonal Antibody
Bcl-W 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot과 ELISA에 적합하며, 미토콘드리아 막 단백질 검출에 유용합니다. Affinity Chromatography로 정제된 액상 형태로 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–193 of human Bcl-W (NP_0040412) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.79 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804695 |
Product Specific Information
- Positive Samples: A-549
- Cellular Location: Mitochondrion membrane, Peripheral membrane protein
Immunogen sequence:
MATPASAPDT RALVADFVGY KLRQKGYVCG AGPGEGPAAD PLHQAMRAAG DEFETRFRRT FSDLAAQLHV TPGSAQQRFT QVSDELFQGG PNWGRLVAFF VFGAALCAES VNKEMEPLVG QVQEWMVAYL ETQLADWIHS SGGWAEFTAL YGDGALEEAR RLREGNWASV RTVLTGAVAL GALVTVGAFF ASK
Target Information
Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRα gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues.
An alternative splicing event in exon 4 results in two transcripts:
- Bcl-w: encodes a protein of 193 amino acids
- Bcl-w/rox: encodes a protein 333 amino acids in length
The rox portion of Bcl-w/rox shows approximately 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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